Nā huahana
Hepps; cep No.: 16052-06-5
Synonyms: Hepps; 16052-06-06-5; Epps; 4- ACID Acid 240-198-8; BRN 3957385; 8JN5PQ99C ACID; EP1 (titration); Hy-D0873 99%; AC-24630; as-69273 acid; n- (hydrxythiyl) pipezine-n'-propane sulfonic acid acid; q15635525; W-107976 acid; 4- (2-hydrnexythyl) pipezine-1-sulfonichonic acid) (Hepps)
ʻO ka waiwai o ka mea kanu o nā hepps
● Nā hiʻohiʻona / kala: keʻokeʻo: keʻokeʻo keʻokeʻo keʻokeʻo
● OLELO MAKENING: 237-239 ° C (Lit.)
● Hoʻohele i nā helu refract: 1.537
● pka: 8.0 (at 25 ℃)
● psa:89.46000
● koi: 1.285 g / cm3
● Logp: -0.16920
● Ke mālama neiʻo Temp.:STRE ma RT.
● solubulity.:h2O: 1 m ma 20 ° C, maopopo, kalaʻole
●ʻO ka wai wai wai.Asolible i ka wai. 25.2 g / l (20 ° C)
● xlogp3: -3.6
● Hōʻike Ho'ōla Na Hydrogen: 2
● Ka heluʻana o ka mea kūʻai aku hydrogen
● Ho'ākoakoa ka Bond Rond: 6
● Ma luna o ka nui: 252.11437830
● helu kūlohelohe: 16
● paʻakikī: 280
ʻO kaʻike safty
● Nā Kiʻi (s):
Xi
● Nā Kūlana Kūlana: Xi
●'ōlelo'ōlelo: 36/37 / 37-22
● Nā'ōlelo palekana palekana: 24 / 25-36-26
Pono
Nā Kūlana Kūʻai:Nā Pāʻani Pūnaewele NITRER -> Pokeazines
Nā Cannical Smiles:C1CN (CCN1CCCS (= O) (= O) O) CCO
Hoʻohana:ʻO EPPS kahi mea maʻamau maʻamau? S Buffer. Ua hoʻohana pinepineʻia ia e like me ka hoʻokaʻawaleʻana i ka ultrathin touping socoring soceling a hoʻonui i ka hopena o nā phosphoglucuase. Hoʻohanaʻia ia e like me keʻano o ka mea kanu i loko o biology a biochemistry.
Hōʻike kiko'ī kiko'ī
E kū anaʻo EPPS no N- (2-Hydroxythyl) pipezine-n '- (3-propanesulfonic Acid). He mea kūʻai akuʻo Zwitterionic i hoʻohana pinepineʻia ma keʻano he mea noiʻi biological a me biochemical.here nui e pili ana i nā epps:
Nā koho waiwai:ʻO EPPS kahi mea kūpono maikaʻi loa e kōkua ai i ka mālamaʻana i kahi PH i nā hoʻonohonoho hoʻokolohua. He waiwai pka o ka poka o 8.09, e hana ia i kūpono no ka buffering i loko o ka ph rapa o 7.0-9.0.
STATIKA:Hōʻikeʻo EPPS i ka holomua maikaʻi ma luna o kahi ākea ākea a emi iki i nā loli i hoʻohālikelikeʻia e nā mea kūʻai'ē aʻe e like me nā mea kanu likeʻole. Hana kēia paʻa i kūpono no ka hoʻohanaʻana i nā hoʻokolohua biochemical a me nā hoʻokolohua nui e koi ai i nā kūlana ph.
Nā noi noi:Hoʻohana pinepineʻia nā EPPS i nā haʻawina aloha likeʻole, e like me ka hoʻomaʻemaʻe ponoʻana, evzyme Assays, a me DNA / RNA / RNA ma kahi. ʻO kona mau kīwī a me ka hoʻomauʻana i ka ph ph ph ph ph ph ph ph ph ph ph ph ph e waiwai ai i kēia mau noi.
Culture Culture:Hiki ke hoʻohanaʻiaʻo EPPS e like me ka mea kanu lāʻau i loko o ka colcture colcture media. Kōkuaʻo ia i ka mālamaʻana i kahi kūlana pH e hiki ai i ka ulu a me ka mālamaʻana i kekahi mauʻano kelepona.
Nāʻae a me ke kūlike:ʻO EPPS kahi hui Zwistic,ʻo ia ka mea i loaʻa i nā uku maikaʻi a maikaʻiʻole. Hiki i kēia hiʻohiʻona ke loaʻa iā ia i nā sweffer maikaʻi loa a me nā hopena maʻamau. Ua kūpono ia me nā molelu koko likeʻole aʻikeʻia e loaʻa i ka hana liʻiliʻi liʻiliʻi me nāʻano likeʻole.
I ka hoʻohanaʻana iā EPPS, he mea nui ia e hahai i nā mea i hoʻopiʻiʻia a me nā alakaʻi alakaʻi PH e hāʻawiʻia e ka mea hana. Eia kekahi, hoʻomaʻamaʻa mau i ka mālamaʻana a hahai i nā protocols palekana palekana kūpono inā e mālama ana i kahi mea hoʻokele a iʻole nā mea hoʻokele.
Noi
Hepps (N- (2-Hydroxyheyl) pipezine-N'-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3-3 - ʻO kekahi o kāna mau noi noi:
Culture Culture: Hoʻohanaʻiaʻo Hepps e like me ka mea kanu kīwī ma ke kahuaʻoihana e mālama ai i kahi PH e mālama i kahi PH a hāʻawi i kahi kūlana kūpono no ka uluʻana a me ka prosultation kūpono. Kōkuaʻo ia i ka hoʻopaʻaʻana i ka PH i loko o keʻano o ka physiological a pale i nā hopena maikaʻi loa ma nā kaʻina hana.
Enzyme Assays:Hoʻohana pinepineʻiaʻo Hepps i kahi buffer ma nā mea hoʻopiʻi enzyme e hoʻonui i nā kūlana i koiʻia no ka hana nui. Hiki i kona mau kekee ke mālama pono i kahi ph o kahi ph, e hōʻoia i nā ana enzerate a hilinaʻi i nā ana.
Mālama ManainHiki ke hoʻohanaʻiaʻo Hepps e like me ka buffer ma nā kaʻina hoʻomaʻemaʻe hoʻomaʻemaʻe. Kōkuaʻo ia i ka mālamaʻana i ke kūpaʻa a me nā hana o nā protein i ka wā hoʻomaʻemaʻe, e like me ke kolamu chromatography a iʻole ka hoʻohanaʻana i ka clamn chromatograph
Nā ElectPophoris:Ua hoʻohanaʻo Hepps i kekahi manawa e like me ka buffer ma Gel Electrophoresis no ka hoʻokaʻawale a me ka nānāʻana i nā proteins a me nā'ōnaehana nucleci. Hiki i kāna PH Buffering hiki ke mālama i kahi pH i kahi ph i ka wā e holo ai ka electrophoretic
Nā Kūlana Kūlana Molelecle:Hiki i nā hepps ke hoʻohanaʻia e like me ka buffer ma nāʻano hana milecular'ē aʻe, me ka PCR (polymerecing, a me nā mea hoʻonaninani. E hōʻoia i kāna mau mea kīwī e hōʻoia i nā kūlana maikaʻi loa no nā hopena kūleʻa a me nā hopena kūpono.
Nā haʻawina e pili ana i nā protein membrane:Ua kūponoʻo Hepps no ke aʻoʻana i nā polokalamu Membrane ma muli o kāna mau haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa. Hoʻohana pinepineʻiaʻo ia ma keʻano he mau hoʻokolohua ma nā hoʻokolohua e pili ana i ka hana hana a iʻole ka hana houʻana i nā polokalamu membrane.
He mea nui ia e hoʻomaopopo i keʻano kūpono a me ka ph of hepps e loli paha ma muli o nāʻano kiko'ī a iʻole nā hanana hoʻokolohua. No laila, he mea kūpono ke kamaʻilioʻana i nā palapala a iʻole nā mea noi no ka hoʻohana ponoʻana a me ka neʻeʻana o nā hepats i kāu mau hoʻokolohua.
