Nā huahana
HEPES paʻakai sodium
Nā huaʻōlelo like: HEPES sodium paʻakai;75277-39-3;HEPES hemisodium paʻakai;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium paʻakai;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium paʻakai;4-(2-Hydroxyethyl)piperazine-1 ethanesulfonic acid ka paʻakai sodium; 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:46758;EBI:46758 278-169-7; 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, paʻakai sodium (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, paʻakai sodium; HEPES sodium paʻakai, >=99.5% (titration);sodium hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) paʻakai sodium;MFCD00036463;HEPES, paʻakai sodium; paʻakai sodium HEPES, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-S.Nalt solution sodium;HEPES; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt; HEPES sodium paʻakai, biochemical papa;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0610868;F2A868; N'-hydroxyethyl-N-piperazineethanesulfonate; HEPES paʻakai sodium, BioXtra, >=99.0% (titration); HEPES paʻakai sodium, Vetec(TM) papa reagent, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium paʻakai;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium paʻakai;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium paʻakai; HEPES sodium paʻakai, BioPerformance Certified, kūpono no ka moʻomeheu cell, >=99.5%
Waiwai Kemika o HEPES Sodium Salt
● Nānā / kala: pauka keʻokeʻo
● Pumi mahu:0Pa ma 25 ℃
● Lae hehee:234 °C
● PKA:7.5(ma 25 ℃)
● PSA：92.29000
● Māmā:1.504[ma 20℃]
● LogP:-0.90190
● Māmā no ka mālama ʻana: Hale kūʻai ma RT.
● Pilikino.:Hygroscopic
● Solubility.:H2O: 1 M ma 20 °C, akaka, kala ole
● Hiki ke hoʻoheheʻe wai.: Hiki ke hoʻoheheʻe ʻia i ka wai.
● Ka helu o ka mea hāʻawi mai i ka Hydrogen Bond:1
● Ka helu o ka mea hoʻokipa hydrogen:6
● Ka helu paʻa hiki ke hoʻololi:5
● Nuipa pololei:260.08067248
● Helu Atoma Kaumaha:16
● Paʻakikī:272
ʻIkepili palekana
● Piktogram(s):
● Nā Code Hazard:
● Nā ʻōlelo palekana: 22-24/25
Pono
Nā Papa Kemika:Nā hoʻohana ʻē aʻe -> Nā mea hoʻolale olaola
ʻO Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Hoʻohana:He kūpono ka paʻakai paʻakai HEPES no nā haʻawina moʻomeheu cell. Hiki ke hoʻohana ʻia i ka hoʻomākaukau ʻana i ka hoʻonā pale o HEPES. Hiki ke hoʻohana ʻia ma ke ʻano he buffer i ka wā o ka phosphorylation assays i nā fibroblasts permeabilized. Hiki ke hoʻohana ʻia no ka haku ʻana i kahi paʻi dialysis paʻakikī, hoʻohana ʻia i ka hoʻoholo ʻana i ka thyroxin manuahi i loko o ka serum e radioimmunoassay. He kūpono ka paʻakai paʻakai HEPES no ka hoʻohana ʻana i nā haʻawina moʻomeheu cell. Hiki ke hoʻohana ʻia i ka hoʻomākaukau ʻana i ka hoʻonā pale o HEPES. Hiki ke hoʻohana ʻia ma ke ʻano he buffer i ka wā o ka phosphorylation assays i nā fibroblasts permeabilized. Hiki ke hoʻohana ʻia no ka haku ʻana i kahi paʻi dialysis paʻakikī, hoʻohana ʻia i ka hoʻoholo ʻana i ka thyroxin manuahi i loko o ka serum e radioimmunoassay.
Hōʻike kikoʻī
HEPES paʻakai sodium, i ʻike ʻia ʻo 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium paʻakai, he ʻano maʻamau o HEPES. ʻO ia ka zwitterionic organic compound e hana ma ke ʻano he buffering agent i ka noiʻi biological a me biochemical.
Hoʻohana pinepine ʻia ka paʻakai paʻakai HEPES i ka media moʻomeheu cell a me nā pale olaola ma muli o kona hiki ke mālama i kahi pae pH paʻa a puni nā kūlana physiological (pH 7.2 - 7.6). Hiki ke hoʻoheheʻe ʻia i ka wai a he haʻahaʻa haʻahaʻa kona ʻona, kūpono ia no nā noi like ʻole.
ʻOi aku ka maikaʻi o ka paʻakai sodium o HEPES i nā noi he nui no ka mea e hoʻonui ai i ka solubility a me ka paʻa, ke hoʻohālikelike ʻia me ka ʻano waikona manuahi. Loaʻa ia ma ke ʻano kūʻai ma ke ʻano he pauka crystalline keʻokeʻo e hiki ke hoʻoheheʻe maʻalahi i ka wai e hoʻomākaukau ai i nā hopena hana.
Hoʻohana maʻamau ka poʻe noiʻi i ka paʻakai sodium HEPES i nā ʻenehana biology molecular, moʻomeheu cell, hoʻāʻo enzyme, hoʻomaʻemaʻe protein, a me nā hoʻokolohua biochemical ʻē aʻe kahi e koʻikoʻi ai ka mālama pH. ʻO kona hiki ke hoʻopaʻa a me ka hoʻohālikelike ʻana me nā ʻōnaehana biological e lilo ia i koho kaulana no ka mālama ʻana i nā kūlana maikaʻi loa no ka hana a me ke kūpaʻa o nā molekala olaola like ʻole.
Ma mua o ka hoʻohana ʻana i ka paʻakai sodium HEPES i kekahi noi kikoʻī, pono ia e nānā i ka palapala, e nīnau i nā ʻōlelo a nā mea hoʻolako, a e hoʻopaʻa i ka pae a me ka pae pH ma muli o nā koi hoʻokolohua kikoʻī.
Palapala noi
Loaʻa ka paʻakai paʻakai HEPES i ka hoʻohana ākea ma nā wahi noiʻi olaola a me ka biochemical. ʻO kekahi o kāna hoʻohana nui ʻana:
Moʻomeheu Cell:Hoʻohui pinepine ʻia ka paʻakai sodium HEPES i ka media moʻomeheu cell no ka mālama ʻana i ka pH paʻa i loko o ka pae physiological a hāʻawi i kahi ʻano kūpono no nā cell e ulu a hoʻonui.
Luna Hoʻopaʻa:Hoʻohana pinepine ʻia ka paʻakai paʻakai HEPES ma ke ʻano he mea hoʻolalelale i nā mea hoʻopaʻa ola a me nā hoʻonā, me ka hoʻāʻo ʻana i ka enzyme, ka hoʻomaʻemaʻe protein, a me nā ʻenehana biology molecular. Kōkua ia i ka mālama ʻana i ka pH mau ma ka pale ʻana i nā loli i ka acidity a i ʻole alkalinity.
Electrophoresis: Hoʻohana ʻia ka paʻakai sodium HEPES ma ke ʻano he pale i ka electrophoresis gel no ka mālama ʻana i ka pH paʻa i ka wā o ka hoʻokaʻawale ʻana o nā waikawa nucleic a i ʻole nā protein. Kāohi ia i ka neʻe ʻana o ka pH i hiki ke hoʻopilikia i ka neʻe a me ka hoʻokaʻawale ʻana o nā molekala i ka gel.
Paʻa Paʻa Protein: Hoʻohui ʻia ka paʻakai paʻakai HEPES i nā hoʻonā protein e hoʻopaʻa i ko lākou ʻano a mālama i kā lākou hana. Kōkua ia i ka mālama ʻana i ka pH maikaʻi loa e pono ai no ka paʻa o ka protein a me ka hana.
Hana Enzyme: Hoʻohana ʻia ka paʻakai sodium HEPES ma ke ʻano he pale i nā hopena enzymatic e mālama i ka pH makemake no ka hana enzyme maikaʻi loa. Kōkua ia e hōʻoia i ka hana ʻana o nā enzyme i kā lākou hana kiʻekiʻe a me ka pololei.
Ke Kiʻi Paʻi Paʻi Ola:Hoʻokomo pinepine ʻia ka paʻakai sodium HEPES i loko o ka media kiʻi no nā hoʻokolohua kiʻi kiʻi ola. ʻO kona hiki ke hoʻopaʻa ʻia e kōkua i ka mālama ʻana i ka pH i makemake ʻia a pale i nā fluctuations i hiki ke hoʻopilikia i ka viability a me ka fluorescence o nā cell.
Nā ʻenehana Biology Molecular: Hoʻohana nui ʻia ka paʻakai sodium HEPES i nā ʻano hana biology molecular, me ka hoʻokaʻawale DNA a i ʻole RNA, PCR, DNA sequencing, a me ka nānā ʻana i ka protein. Hāʻawi kona hiki ke hoʻopaʻa i nā hopena pololei a hilinaʻi i kēia mau kaʻina hana.
He mea nui e hoʻomaopopo he ʻokoʻa paha ka hoʻohana ʻana a me ka neʻe ʻana o ka paʻakai sodium HEPES ma muli o nā koi hoʻokolohua a me ka ʻōnaehana olaola e aʻo ʻia nei. No laila, ʻoi aku ka maikaʻi e nīnau i nā palapala a me nā ʻōlelo aʻoaʻo no ka hoʻohana pono ʻana.
